Characterization of newly isolated cellulose-degrading bacteria Bacillus licheniformis CMC1 and Bacillus sonorensis JRM36
- Characterization of newly isolated cellulose-degrading bacteria Bacillus licheniformis CMC1 and Bacillus sonorensis JRM36
- 강금래; 김은숙; 우한민; 김경헌; 엄영순
- cellulosic biomass; bacillus; 2,3-butanediol; acetoin
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- Cellulosic biomass is the most abundant renewable bioresource for the production of bio-based products and bioenergy. Recent studies focus on hydrolyzing cellulosic biomass more economically for biorefinery process and consolidated bioprocessing (CBP) by cellulolytic microorganisms has been proposed. In this study, two cellulolytic bacteria were isolated from soil in Indonesia and Jiri mountain in Republic of Korea. These isolates, CMC1 and Jirisan36, were identified as members of the genus Bacillus by analysis of the 16S rRNA gene sequence, showing 99.7% similarity to Bacillus licheniformis and 99.7% similarity to Bacillus sonorensis, respectively. B. licheniformis CMC1 produced 2,3-butanediol and B. sonorensis Jirisan36 produced acetoin as a main product under aerobic conditions using various carbon sources such as glucose, cellobiose and xylose. Both of strains showed cellulolytic activity on agar plates containing cellulose by Congo red staining method. The total reducing sugar concentration increased continuously and cellulase activity was also detected in the supernatant during the incubation on a cellulose medium. These data suggest that B. licheniformis CMC1 and B. sonorensis Jirisan36 possess potentials for 2,3-butanol and acetoin from cellulose.
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