Alteration of Bile Acid Metabolism in Pseudo Germ-Free Rats
- Alteration of Bile Acid Metabolism in Pseudo Germ-Free Rats
- 사릴꾸마르; 안지혜; 이수현; 정병화
- Pseudo germ-free rat; Oxysterol; Liver toxicity; Bile acids; Gas chromatography-mass spectrometry
- Issue Date
- Archives of pharmacal research
- VOL 35, NO 11, 1969-1977
- To characterize the impact of gut microbiota on host bile acid metabolism, we investigated the
metabolic profiles of oxysterols and bile acids (BAs) in a conventional rat model (SD) (n=5) and
its pseudo germ-free (GF) equivalent (n=5). GF rats were developed by the oral administration
of bacitracin, neomycin and streptomycin (200 mg/kg, each) twice a day for 6 days. Urinary levels
of oxysterols and bile acid metabolites were quantified using gas chromatography-mass spectrometry
(GC-MS). The activity levels of enzymes involved in the bile acid metabolic pathway
were determined through urinary concentration ratio between product to precursor. Cholic acid
(CA) and α-/β-muricholic acid (α-/β-MCA) were significantly elevated at pseudo germ-free condition.
An increase of hydroxylase (cholesterol 7α-hydroxylase, oxysterol 7α-hydroxylase and cytochrome
P450 scc) and a significant decrease of 7α-dehydroxylase were observed. The urinary
concentration ratio of primary bile acids, a marker for hepatotoxicity, increased in pseudo germfree
conditions. Therefore, it was found that gut microbiota could play a significant role in the
bile acids homeostasis and metabolism.
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