IMPROVED TOLERANCE OF THE CELLULAR TOXICITY FROM LIGNOCELLULOSIC HYDROLYSATE IN ENGINEERED Corynebacterium glutamicum
- IMPROVED TOLERANCE OF THE CELLULAR TOXICITY FROM LIGNOCELLULOSIC HYDROLYSATE IN ENGINEERED Corynebacterium glutamicum
- 이정석; 박홍실; 엄영순; 오민규; 우한민
- Corynebacterium glutamicum; furfural
- Issue Date
- 한국화학공학회 2013년도 봄 총회 및 학술대회
- Acid-pretreatment of lignocellulosic biomasses produced fermentable sugars and cellular inhibitors such as Furfural, HMF, etc. We developed the strain capable of tolerant to the inhibitors. Initially, we focused the effect of the inhibitors to C. glutamicum. Cellular growth was retarded under various conditions of cellular inhibitors, including furfural that is a derivative of furan in the hydrolysate.
C. glutamicum grown with furfural showed over 2-folds of reactive oxygen species stresses. Moreover, the conversion rate of furfural was calculated. We have engineered C. glutamicum by overexpressing the target genes. When we cultivated the engineered strains with furfural, they showed improved the growth rate. Several metabolic engineering approaches could further improve the tolerance in C. glutamicum. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (2013, University-Institute cooperation program) and ‘Creative Allied Program (CAP)’ through the Korea Research Council of Fundamental Science and Technology (KRCF) and Korea Institute of Science and Technology (KIST).
- Appears in Collections:
- KIST Publication > Conference Paper
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.