IMPROVED TOLERANCE OF THE CELLULAR TOXICITY FROM LIGNOCELLULOSIC HYDROLYSATE IN ENGINEERED Corynebacterium glutamicum

Title
IMPROVED TOLERANCE OF THE CELLULAR TOXICITY FROM LIGNOCELLULOSIC HYDROLYSATE IN ENGINEERED Corynebacterium glutamicum
Authors
이정석박홍실엄영순오민규우한민
Keywords
Corynebacterium glutamicum; furfural
Issue Date
2013-04
Publisher
한국화학공학회 2013년도 봄 총회 및 학술대회
Abstract
Acid-pretreatment of lignocellulosic biomasses produced fermentable sugars and cellular inhibitors such as Furfural, HMF, etc. We developed the strain capable of tolerant to the inhibitors. Initially, we focused the effect of the inhibitors to C. glutamicum. Cellular growth was retarded under various conditions of cellular inhibitors, including furfural that is a derivative of furan in the hydrolysate. C. glutamicum grown with furfural showed over 2-folds of reactive oxygen species stresses. Moreover, the conversion rate of furfural was calculated. We have engineered C. glutamicum by overexpressing the target genes. When we cultivated the engineered strains with furfural, they showed improved the growth rate. Several metabolic engineering approaches could further improve the tolerance in C. glutamicum. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (2013, University-Institute cooperation program) and ‘Creative Allied Program (CAP)’ through the Korea Research Council of Fundamental Science and Technology (KRCF) and Korea Institute of Science and Technology (KIST).
URI
http://pubs.kist.re.kr/handle/201004/44805
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KIST Publication > Conference Paper
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