Quantitative analysis of organochlorine pesticides in human serum using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry

Title
Quantitative analysis of organochlorine pesticides in human serum using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry
Authors
김미옥송나래홍종기이정애표희수
Keywords
Organochlorine pesticides; HS-SPME; GC-MS; Human serum; Korea
Issue Date
2013-07
Publisher
Chemosphere
Citation
VOL 92, NO 3, 279-285
Abstract
Persistent organochlorine pesticides (OCPs) are well known to accumulate in environment and to instigate disruptions in endocrine system. In the present study, 18 kinds of OCPs were bio-monitored from 1904 adult serums. Analysis was performed with headspace gas chromatography (HS-GC) which has the advantage to require small volume of serum (1 mL) without complex pretreatment and 13C isotope labeled internal standard mix was used for quantitation. As a result, eleven out of 18 OCPs, including hexachlorobenzene (HCB) and dichlorodiphenyltrichloroethane metabolites (DDTs) were detected. HCB was the most frequently detected OCP (8.0%) with an average concentration of 1.74 ng g−1 lipid (2.10 ng g−1 lipid–99.80 ng g−1 lipid). p,p′-Dichlorodiphenyldichloroethylene (p,p′-DDE) had the highest average concentration of 21.65 ng g−1 lipid (12.80 ng g−1 lipid–2270 ng g−1 lipid) and was detected from 7.5% of serum samples. The average concentration of ∑DDT metabolites was 32.04 ng g−1 lipid (6.61 ng g−1 lipid–2800 ng g−1 lipid) with detection frequency of 9.0%. Results interpreted with respect to age groups showed increase in ∑OCPs and ∑DDTs concentrations as age increases, except for the 20. Results interpreted with respect to gender, in general, illustrated higher detected concentration of OCPs in male serums than in female serums; however, the result trend was reversed in age group of the sixties. The above results will be applicable to health impact assessment of Koreans in relation to environmental contaminations.
URI
http://pubs.kist.re.kr/handle/201004/44870
ISSN
00456535
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KIST Publication > Article
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