Determination of total mercury and methyl mercury in biological fluids by utilizing DMA and GC-MS

Title
Determination of total mercury and methyl mercury in biological fluids by utilizing DMA and GC-MS
Authors
정선아김승기문명희표희수
Issue Date
2013-05
Publisher
한국분석과학회
Abstract
Mercury exists as an element, inorganic mercury and organic mercury in nature. Methyl mercury is known as typical form of organic mercury and easily absorbed into the body and has a longer half-life in vivo. In this study, total mercury (T-Hg) and methyl mercury (Me-Hg) were analyzed in biological fluids for investigation of the correlation between T-Hg and Me-Hg. Biological samples (n=4000) were divided in four groups, according to T-Hg concentration: group I (25percentile - 50percentile), group II (50percentile - 75percentile), group III (75percentile - 95percentile) and group IV (95percentile - 100percentile). 100 samples were randomly selected from each group. Followed blood Me-Hg, urine T-Hg and urine Me-Hg were measured. T-Hg was analyzed by using Direct Mercury Analyzer (DMA), and obtained limit of detection (LOD) was 0.2 μgL-1. Me-Hg in blood was analyzed with ethylate derivatization using headspace-GC-MS, and obtained LOD was 0.5 μgL-1. Me-Hg in urine was analyzed by using LLE and GC-MS, and obtained LOD was 0.2 μgL-1. T-Hg and Me-Hg were detected from the all blood sample (n=400). The geometric means of blood T-Hg and blood Me-Hg were 6.35 μgL-1 and 4.44 μgL-1, respectively, and 71.91 % of blood T-Hg was presented as blood Me-Hg. The fraction of blood Me-Hg in blood T-Hg is generally similar, but female were significantly higher than male (p<0.05). T-Hg was detected from 85% of urine samples (n=200), and obtained geometric mean was 0.72 μgL-1. Me-Hg was not detected from the all urine samples (n=200).
URI
http://pubs.kist.re.kr/handle/201004/44919
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KIST Publication > Conference Paper
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