Determination of total mercury and methyl mercury in biological fluids by utilizing DMA and GC-MS
- Determination of total mercury and methyl mercury in biological fluids by utilizing DMA and GC-MS
- 정선아; 김승기; 문명희; 표희수
- Issue Date
- Mercury exists as an element, inorganic mercury and organic mercury in nature. Methyl mercury is known as typical form of organic mercury and easily absorbed into the body and has a longer half-life in vivo.
In this study, total mercury (T-Hg) and methyl mercury (Me-Hg) were analyzed in biological fluids for investigation of the correlation between T-Hg and Me-Hg. Biological samples (n=4000) were divided in four groups, according to T-Hg concentration: group I (25percentile - 50percentile), group II (50percentile - 75percentile), group III (75percentile - 95percentile) and group IV (95percentile - 100percentile). 100 samples were randomly selected from each group. Followed blood Me-Hg, urine T-Hg and urine Me-Hg were measured. T-Hg was analyzed by using Direct Mercury Analyzer (DMA), and obtained limit of detection (LOD) was 0.2 μgL-1. Me-Hg in blood was analyzed with ethylate derivatization using headspace-GC-MS, and obtained LOD was 0.5 μgL-1. Me-Hg in urine was analyzed by using LLE and GC-MS, and obtained LOD was 0.2 μgL-1.
T-Hg and Me-Hg were detected from the all blood sample (n=400). The geometric means of blood T-Hg and blood Me-Hg were 6.35 μgL-1 and 4.44 μgL-1, respectively, and 71.91 % of blood T-Hg was presented as blood Me-Hg. The fraction of blood Me-Hg in blood T-Hg is generally similar, but female were significantly higher than male (p<0.05). T-Hg was detected from 85% of urine samples (n=200), and obtained geometric mean was 0.72 μgL-1. Me-Hg was not detected from the all urine samples (n=200).
- Appears in Collections:
- KIST Publication > Conference Paper
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.