Analysis of bioactive components in blueberry, raspberry and blackberry based on metabolmics

Analysis of bioactive components in blueberry, raspberry and blackberry based on metabolmics
metabolomics; berry
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Blueberry (Vaccinium corymbosum L.), Raspberry (Rubus idaeus L.) and Blackberry (Rubus sp.), widely cultivated in the world, and berries were processed in functional health foods such as beverages, yogurts, and jam. In this study, we identified components of blueberry, raspberry and blackberry using gas chromatography-mass spectrometer (GC-MS) and mass profiler professional. Mass spectrometry based metabolite profiling in combination with multivariate data analysis was introduced to quantity changes in metabolic patterns. Blueberry, raspberry and blackberry were sequentially extracted with hexane, ethyl ether, ethyl acetate and methanol. The extracted berries were evaporated to dryness, derivatized with MSTFA and then injected on GC-MS. The data of whole component of berries were processed by principle component analysis (PCA) as multivariate analysis to determine whether separate classes of extraction solvents. PCA score plot obtained from hexane ethyl ether, ethyl acetate, and methanol extracts was the good discrimination between berries. And then, loading plot analysis performed to increase accuracy of data processing was collected 89 peaks. Among them, 27 peaks of main component were categorized in the significant level using ANOVA test (p<0.001). Although we have not yet identified the component of berries, we found some phytochemicals such as oleic acid and citric acid. Oleic acid from berries extract was known as health food to decreasing heart disease. And citric acid has functions of anti-aging and detoxification.
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