A simple colorimetric assay for the detection of mercury ions based-on the interaction between mercyry ion specific ssDNA and catalytic magnetic nanoparticles

Title
A simple colorimetric assay for the detection of mercury ions based-on the interaction between mercyry ion specific ssDNA and catalytic magnetic nanoparticles
Authors
김연석진성민김병찬정종수
Keywords
colorimetric assay; mercury; ssDNA; aptamer
Issue Date
2013-05
Publisher
3rd International Confenrece on Bio-sensing Technology
Abstract
We developed a simple colorimetric assay for the detection of metal ions based on the peroxidase-like activity of magnetic nanoparticles (MNPs) and their interactioin with target specific single-stranded DNA (ssDNA). It was already known that MNPs easily oxidize substrates in the presence of hydrogen peroxide same as a peroxidase. In this study, the target specific ssDNA was adsorbed on the positively charged surface of MNP, then the catalytic activity of MNPs was reduced by the screening effect of adsorbed ssDNA on the surface of MNPs to substrates. The peroxidase like activity of MNPs was recovered by the addition of targets because the target specific ssDNA were displaced from MNPs by the interaction between targets and their specific ssDNA. The degree of color change of mixture was proportional to the concentration of mercury ion in a range of 5 to 75 μM. In control experiment, other metal ions did not induce the color change.
URI
http://pubs.kist.re.kr/handle/201004/45005
Appears in Collections:
KIST Publication > Conference Paper
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