Continuous Monitoring of Hazardous Pollutants in Water using Receptor Immobilized Porous Membrane System
- Continuous Monitoring of Hazardous Pollutants in Water using Receptor Immobilized Porous Membrane System
- 김연석; 김병찬; 정종수
- Continuous monitoring; Receptor; Porous membrane; Aptamer
- Issue Date
- 한국바이오칩학회 춘계학술발표대회
- Continuous monitoring of hazardous pollutants in water is very important for water quality control and management. However, almost of detection and analysis methods are discontinuous systems. In this report, we suggested the continuous monitoring method of toxic materials using receptor immobilized porous membrane. As a model system, Hg2+ specific ssDNA (M-DNA) and mercury ion was adopted. Firstly, quencher-dye labelled M-DNA was hybridized with fluorescence-dye and amino group labelled complementary ssDNA (C-DNA), which resulted in quenching of fluorescence. This duplex form of DNA receptor was immobilized on COOH-functionalized porous silicate membrane. The sample containing Hg2+ was continuously flowed through receptor immobilized silicate membrane. The Hg2+ led to release the M-DNA from duplex DNA receptor by interaction with M-DNA, subsequently the fluorescence from C-DNA on membrane was recovered and accumulated. The fluorescence intensity of membrane was consistently measured by imaging analysis. As a result, the degree of fluorescence increasing was proportional to Hg2+ concentration and reverse proportional to flow rate of sample. A flowing of just water or other heavy metal ions did not increase the fluorescence signal. To perform the continuous monitoring of Hg2+, the time dependant accumulated fluorescence signal was differentiated, and the differentiation data was well matched to the concentration of Hg2+ at each time.
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