Detection of E. coli using Aptamer Cocktail Newly Isolated against E. coli
- Detection of E. coli using Aptamer Cocktail Newly Isolated against E. coli
- 김연석; 송민영; 정종수; 김병찬
- Aptamer cocktail; E. coli; detection; electrochemical analysis
- Issue Date
- 2013 Internatinoal Environmental Engineering Conference
- Contamination of Escherichia coli (E. coli) in water is a critical problem for public health and safety because some E. coli strains, such as E. coli O157:H7, are pathogenic. Even though most of the E. coli strains found in water are not virulent, E. coli is good indicator of microbial water contamination because the existence of E. coli indicates that microbial contamination is high1). Therefore, detection of E. coli is a major issue for quality control of water. The conventional culture-based method for E. coli detection is powerful and cheap; however, it usually takes 2–3 days to obtain a result, which does not permit rapid on-site detection of E. coli us to develop.
In this study, we isolated DNA aptamers to E. coli using a whole bacterial cell-SELEX process and characterized their affinity and selectivity to other E. coli strains and fecal coliform bacteria cells. The aptamer-based E. coli detection was performed by fluorescence analysis and its sensitivity enhaced by usage of cocktail mixture of E. coli binding aptamers.
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