Detection of E. coli using Aptamer Cocktail Newly Isolated against E. coli

Detection of E. coli using Aptamer Cocktail Newly Isolated against E. coli
Aptamer cocktail; E. coli; detection; electrochemical analysis
Issue Date
2013 Internatinoal Environmental Engineering Conference
Contamination of Escherichia coli (E. coli) in water is a critical problem for public health and safety because some E. coli strains, such as E. coli O157:H7, are pathogenic. Even though most of the E. coli strains found in water are not virulent, E. coli is good indicator of microbial water contamination because the existence of E. coli indicates that microbial contamination is high1). Therefore, detection of E. coli is a major issue for quality control of water. The conventional culture-based method for E. coli detection is powerful and cheap; however, it usually takes 2–3 days to obtain a result, which does not permit rapid on-site detection of E. coli us to develop. In this study, we isolated DNA aptamers to E. coli using a whole bacterial cell-SELEX process and characterized their affinity and selectivity to other E. coli strains and fecal coliform bacteria cells. The aptamer-based E. coli detection was performed by fluorescence analysis and its sensitivity enhaced by usage of cocktail mixture of E. coli binding aptamers.
Appears in Collections:
KIST Publication > Conference Paper
Files in This Item:
There are no files associated with this item.
RIS (EndNote)
XLS (Excel)


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.