Novel lignin-degrading Burkholderia strains from forest soil

Novel lignin-degrading Burkholderia strains from forest soil
Issue Date
World Biotechnology Congress 2013
Two novel aerobic strains, designated as JRM1-1 and JRM2-1 were isolated from the forest soil of Jirisan Mountain, Korea. Screening for lignin-degrading bacteria was performed using Azure B dye medium and Azure B dye-decolorizing strains were regarded as lignin-degrading strain candidates. Based on 16S rRNA gene sequence similarity, the closest relatives of JRM1-1 and JRM2-1 were Burkholderia phytofirmans PsJNT (98.4%) and Burkholderia terrae KMY02T (97.2%), respectively. These strains were also known as plant-associated bacteria with plant-beneficial properties. The isolates were characterized based on the modern polyphasic taxonomy. The phylogenetic analysis and characteristics of the strains JRM1-1 and JRM2-1 showed that both strain JRM1-1 and JRM2-1 were clearly the member of the genus Burkholderia. Lignin-degrading activity of the strains JRM1-1 and JRM2-1 were further tested using veratryl alcohol assay which is the most widely used method in screening enzyme activity of the cleavage of β-O-4 linkage in lignin. The strains were cultivated for 5 days using YPG medium and the supernatant was used for veratryl alcohol assay. As a result, the oxidation of veratryl alcohol to veratryl aldehyde was confirmed using the supernatant of the cultures, indicating those strains are likely to be lignin-degrading microorganism candidates.
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