Development of the Jet-fuel production using engineered Corynebacterium glutamicum using a CoryneBrick platform
- Development of the Jet-fuel production using engineered Corynebacterium glutamicum using a CoryneBrick platform
- 강민경; 엄영순; 심상준; 우한민
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- The production of alternative biofuels has been developed due to rising petroleum costs and environment concerns. Currently, biological engineering for the production of chemicals and fuels have been developed and implemented in engineered microbes. Corynebacterium glutamicum, a widely-known industrial bacterium, was engineered by introducing biofuel producing pathway constructed in the BioBrick-formatted expression vector systems. CoryneBricks, E. coli-C .glutamicum shuttle vector, were constructed with different origins of replication and controllable promoters. CoryneBrick vectors provide useful tool for users to choose the appropriate vectors in Corynebacterium and were applied to reconstruct the pinene biosynthetic pathway for the jet fuel production. A target jet-fuel precursor is further converted to high density jet-fuels via catalytic processes. So, the jet-fuel production platform by engineered C. glutamicum could be useful to biofuels production application. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (2013, University-Institute cooperation program) and ‘Creative Allied Program (CAP)’ through the Korea Research Council of Fundamental Science and Technology (KRCF) and Korea Institute of Science and Technology (KIST).
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