Bacteriophages as Templates for Manufacturing Supramolecular Structures

Title
Bacteriophages as Templates for Manufacturing Supramolecular Structures
Authors
Korkmaz, NuriyeKim, Young JunNam, Chang Hoon
Keywords
apatite-like materials; biomineralization; electron microscopy; metallization; phage bundles
Issue Date
2013-03
Publisher
Macromolecular bioscience
Citation
VOL 13, NO 3, 376-387
Abstract
SS phages are genetically enginnered by replacing 2 N-terminal amino acids of the p8 coat protein of the fd phage. AGE and zeta potential measurements show that SS phages carry at least 1/4 less net negative surface charge than fd phages. Morphology and thickness of phages are studied at different counterion concentrations (10-3, 10-2 and 10-1 M) by AFM, SEM and immunofluorescence analyses. Bundles induced by CoCl2 and CaCl2 are either metallized by chemical reduction or biomineralized for apatite-like material formation. EDX spectroscopy confirms the presence of Co, P and Ca peaks in mineralized samples. Such bottom-up manufactured phage scaffolds might be applied in bioengineering studies.
URI
http://pubs.kist.re.kr/handle/201004/45152
ISSN
16165187
Appears in Collections:
KIST Publication > Article
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