Fast spore breaking by superheating

Title
Fast spore breaking by superheating
Authors
Pribylka, AdamAlmeida, Ana VanessaAltmeyer, Matthias O.Petr, JanSevcik, JurajManz, AndreasNeuzil, Pavel
Issue Date
2013-05
Publisher
Lab on a chip
Citation
VOL 13, NO 9, 1695-1698
Abstract
Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120–180 ℃. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 ℃ resulted in the release of DNA within 20 s.
URI
http://pubs.kist.re.kr/handle/201004/45155
ISSN
14730197
Appears in Collections:
KIST Publication > Article
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