Fast spore breaking by superheating
- Fast spore breaking by superheating
- Pribylka, Adam; Almeida, Ana Vanessa; Altmeyer, Matthias O.; Petr, Jan; Sevcik, Juraj; Manz, Andreas; Neuzil, Pavel
- Issue Date
- Lab on a chip
- VOL 13, NO 9, 1695-1698
- Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was
pumped through the open-ended capillary tube mounted across the heated zone. We investigated the
influence of temperature in the range 120–180 ℃. The heat exposure was controlled by the length of the
heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment
above 120 ℃ resulted in the release of DNA within 20 s.
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