Multiple Products Monitoring as a Robust Approach for Peptide Quantification
- Multiple Products Monitoring as a Robust Approach for Peptide Quantification
- 백제현; 김호근; 신병희; 유명희
- peptide quantification; product ion monitoring; multiple products monitoring, target
- Issue Date
- Journal of proteome research
- VOL 8, NO 7, 3625-3632
- Quantification of target peptides and proteins is crucial for biomarker discovery. Approaches such as
selected reaction monitoring (SRM) and multiple reaction monitoring (MRM) rely on liquid chromatography
and mass spectrometric analysis of defined peptide product ions. These methods are not
very widespread because the determination of quantifiable product ion using either SRM or MRM is a
very time-consuming process. We developed a novel approach for quantifying target peptides without
such an arduous process of ion selection. This method is based on monitoring multiple product ions
(multiple products monitoring: MpM) from full-range MS2 spectra of a target precursor. The MpM
method uses a scoring system that considers both the absolute intensities of product ions and the
similarities between the query MS2 spectrum and the reference MS2 spectrum of the target peptide.
Compared with conventional approaches, MpM greatly improves sensitivity and selectivity of peptide
quantification using an ion-trap mass spectrometer.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.