The Chemopreventive Effects of Carpesium abrotanoides Are Mediated by Induction of Phase II Detoxification Enzymes and Apoptosis in Human Colorectal Cancer Cells

Title
The Chemopreventive Effects of Carpesium abrotanoides Are Mediated by Induction of Phase II Detoxification Enzymes and Apoptosis in Human Colorectal Cancer Cells
Authors
이샛별강경수이희주윤지호조은혜김철영노주원
Keywords
apoptosis; carpesium abrotanoides; colon cancer; Nrf2; quinone reductase; NF-E2-related factor 2
Issue Date
2010-02
Publisher
Journal of medicinal food
Citation
VOL 13, NO 1, 39-46
Abstract
Cancer chemoprevention is thought to occur either by blocking the initiation of or suppressing the promotion of carcinogenesis. Phase II detoxification enzymes are known to play important roles in cancer chemoprevention because they enhance cytoprotection through detoxification and elimination of activated carcinogens at tumor initiation. Apoptosis is one of the most important inhibitory targets for tumor promotion. In this study, we have investigated the cancer chemopreventive activity of the ethanolic extract of Carpesium abrotanoides (CAE). We found that CAE induced quinone reductase [also known as NAD(P)H:quinone oxidoreductase] activity, increased NAD(P)H:quinone oxidoreductase mRNA and protein expression, and had a relatively high chemoprevention index (12.04). CAE also significantly activated the antioxidant response element through the nuclear accumulation of NF-E2-related factor 2 in HCT116. Interestingly, we also found that CAE induced apoptosis, as evidenced by the externalization of phosphatidylserine, increased sub-G0/G1 content, chromatin condensation, poly(ADP-ribose) polymerase cleavage, and p53. These data suggest that the chemopreventive effects of C. abrotanoides can result from both the induction of phase II detoxification enzymes and from apoptosis. Thus, CAE could potentially be developed as a cancer chemopreventive agent for prevention or treatment of human cancers.
URI
http://pubs.kist.re.kr/handle/201004/45558
ISSN
1096-620X
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