2-layer based microfluidic concentration generator by hybrid serial and volumetric dilutions

Title
2-layer based microfluidic concentration generator by hybrid serial and volumetric dilutions
Authors
이강선김충김영은정근휘안병욱강지윤오광욱
Keywords
Serial dilution; Volumetric dilution; Drug screening; Microfluidic network
Issue Date
2010-04
Publisher
Biomedical microdevices
Citation
VOL 12, NO 2, 297-309
Abstract
We present a 2-layer based microfluidic concentration generator by a hybrid of a serial and a volumetric dilution for dose-response experiments in drug screening. The hybrid dilution method using 2-layer based microfluidic network significantly reduces the total number of cascaded serial dilution stages. The proposed strategy is capable of generating a large number of universal stepwise monotonic concentrations with a wide range of logarithmic and linear scales. We have studied an equivalent electrical circuit to that of the 2-layer based microfluidic network, where the only variable parameter is channel length. We have designed a microfluidic dilution generator simultaneously covering 14 doses with a combination of 4-order logarithmic and 4-point linear concentrations. The design has been verified by a commercial circuit analysis software (e.g., P-Spice) for the electrical circuit analysis and a computational fluid dynamics software (e.g., CFD-ACE+) for the microfluidic circuit analysis. As a real-life application of the proposed dilution generator, we have successfully performed a dose-response experiment using MCF-7 human breast cancer cells. We expect that the proposed dilution method will be useful to study not only high throughput drug screening but also optimization in biology, chemistry, medicine, and material sciences.
URI
http://pubs.kist.re.kr/handle/201004/45570
ISSN
1387-2176
Appears in Collections:
KIST Publication > Article
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