Nanoscale enzyme reactors in mesoporous carbon for improved performance and lifetime of biosensors and biofuel cells

Title
Nanoscale enzyme reactors in mesoporous carbon for improved performance and lifetime of biosensors and biofuel cells
Authors
권기영윤종규김재현박영진전철민김병찬권영채Xueyan ZhaoePing Wange상병인이진우Hyun Gyu ParkHo Nam ChangTaeghwan HyeonSu HaHee-Tae Jung김중배
Keywords
Biofuel cells; Biosensors; Enzyme crosslinking; Mesoporous carbon; Nanoscale enzyme reactors
Issue Date
2010-10
Publisher
Biosensors & bioelectronics
Citation
VOL 26, NO 2, 655-660
Abstract
Nanoscale enzyme reactors (NERs) of glucose oxidase in conductive mesoporous carbons were prepared in a two-step process of enzyme adsorption and follow-up enzyme crosslinking. MSU-F-C, a mesoprous carbon, has a bottleneck pore structure with mesocellular pores of 26nm connected with window mesopores of 17 nm. This structure enables the ship-in-a-bottle mechanism of NERs, which effectively prevents the crosslinked enzymes in mesocellular pores from leaching through the smaller window mesopores. This NER approach not only stabilized the enzyme but also expedited electron transfer between the enzyme and the conductive MSU-F-C by maintaining a short distance between them. In a comparative study with GOx that was simply adsorbed without crosslinking, the NER approach was proven to be effective in improving the sensitivity of glucose biosensors and the power density of biofuel cells. The power density of biofuel cells could be further improved by manipulating several factors, such as by adding a mediator, changing the order of adsorption and crosslinking, and inserting a gold mesh as an electron collector.
URI
http://pubs.kist.re.kr/handle/201004/45595
ISSN
0956-5663
Appears in Collections:
KIST Publication > Article
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