Decellularized PLGA-based scaffolds and their osteogenic potential with bone marrow stromal cells

Title
Decellularized PLGA-based scaffolds and their osteogenic potential with bone marrow stromal cells
Authors
홍유진배순언도선희김익환한동근박귀덕
Keywords
bone marrow stromal cells; osteogenic differentiation; extracellular matrix; scaffold; decellularization
Issue Date
2011-10
Publisher
Macromolecular research
Citation
VOL 19, NO 10, 1090-1096
Abstract
A cell-derived extracellular matrix (ECM) was naturally obtained and its effect on the induction of osteogenesis of bone marrow stromal cells (BMSCs) was investigated. Once porous composite scaffolds made of poly(Llactic- co-glycolic acid) (PLGA), hydroxyapatite (HA), and β-tricalcium phosphate (β-TCP) were fabricated, these scaffolds were seeded with fibroblasts or preosteoblasts and cultured in vitro. They were then subjected to decellularization, resulting in fibroblasts-decellularized scaffolds (FDS) or preosteoblasts-decellularized scaffolds (PDS). Both fibronectin and type I collagen were clearly detected from the immunofluorescent staining of FDS and PDS, respectively. When the rabbit BMSCs-loaded scaffolds were cultured in the osteogenic medium for 4 weeks, the osteogenic potential of FDS and PDS was much greater than the PLGA/HA/β-TCP (control), as identified by histological staining and the alkaline phosphatase (ALP) activity. Meanwhile, when BMSC-seeded FDS was implanted subcutaneously into the nude mice, the results also indicated more upregulated osteogenic differentiation of BMSCs in vivo compared to the control. This study suggests that the microenvironment created by cell-derived ECM can provide a favorable template in prompting the osteogenesis of BMSCs.
URI
http://pubs.kist.re.kr/handle/201004/45631
ISSN
1598-5032
Appears in Collections:
KIST Publication > Article
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