Decellularized PLGA-based scaffolds and their osteogenic potential with bone marrow stromal cells
- Decellularized PLGA-based scaffolds and their osteogenic potential with bone marrow stromal cells
- 홍유진; 배순언; 도선희; 김익환; 한동근; 박귀덕
- bone marrow stromal cells; osteogenic differentiation; extracellular matrix; scaffold; decellularization
- Issue Date
- Macromolecular research
- VOL 19, NO 10, 1090-1096
- A cell-derived extracellular matrix (ECM) was naturally obtained and its effect on the induction of osteogenesis
of bone marrow stromal cells (BMSCs) was investigated. Once porous composite scaffolds made of poly(Llactic-
co-glycolic acid) (PLGA), hydroxyapatite (HA), and β-tricalcium phosphate (β-TCP) were fabricated, these
scaffolds were seeded with fibroblasts or preosteoblasts and cultured in vitro. They were then subjected to decellularization,
resulting in fibroblasts-decellularized scaffolds (FDS) or preosteoblasts-decellularized scaffolds (PDS).
Both fibronectin and type I collagen were clearly detected from the immunofluorescent staining of FDS and PDS,
respectively. When the rabbit BMSCs-loaded scaffolds were cultured in the osteogenic medium for 4 weeks, the
osteogenic potential of FDS and PDS was much greater than the PLGA/HA/β-TCP (control), as identified by histological
staining and the alkaline phosphatase (ALP) activity. Meanwhile, when BMSC-seeded FDS was implanted
subcutaneously into the nude mice, the results also indicated more upregulated osteogenic differentiation of BMSCs
in vivo compared to the control. This study suggests that the microenvironment created by cell-derived ECM can
provide a favorable template in prompting the osteogenesis of BMSCs.
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