Compound Isolated from the Leaves of Phyllostachys nigra Protects NMDA-Induced Retinal Degeneration
- Compound Isolated from the Leaves of Phyllostachys nigra Protects NMDA-Induced Retinal Degeneration
- 김경아; 강규동; 조형; 이희주; 김철영; 엄병헌; 정상훈
- Glaucoma; Phyllostachys nigra; NMDA; RGC-5
- Issue Date
- Journal of food biochemistry
- VOL 37, NO 4, 465-474
- The luteolin 6-C-(6″-O-trans-caffeoylglucoside) (PN6) isolated from Phyllostachys
nigra is effective against both the negative influence of N-methyl-Daspartate
(NMDA) to the rat retina and the oxidative stress induced transformed
retinal ganglion cells (RGC-5) death.
The PN6 concentration-dependently inhibited sodium nitroprusside-induced
lipid peroxidation. Treatment of the RGC-5 with PN6 decreased the apoptotic
proteins of poly (adenosine diphosphate-ribose) polymerase (PARP) and cleaved
caspase-3, and increased the antioxidant proteins of superoxide dismutase
(SOD)-2, catalase and glutathione peroxidase (GPx-1) expressions by Western
blot analysis. The PN6 reduced the thickness of the inner plexiform layer using
hematoxylin and eosin staining and decreased the number of terminal deoxynucleotidyl
transferase 2′-deoxyuridine 5′-triphosphate nick-end labeling
(TUNEL)-positive cells using TUNEL kit assay. Moreover, PN6 attenuated
upregulation of apoptotic proteins (PARP and cleaved caspase-3) and downregulation
of antioxidant proteins (SOD-2, catalase and GPx-1) caused by NMDA in
the rat retina.
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