Enhanced extraction efficiency of miRNA from cells by addition of Triton X-100
- Enhanced extraction efficiency of miRNA from cells by addition of Triton X-100
- 반은미; 채동규; 송은주
- microRNA; Triton X-100; extraction efficiency
- Issue Date
- Analytical and bioanalytical chemistry
- VOL 405, NO 23, 7535-7539
- The determination of microRNA (miRNA) levels
in biomaterials has become important for understanding their
biological functions and for the diagnosis of various diseases.
An effective extraction method is needed for maximizing
the recovery of miRNAs from cells, while minimizing
RNA degradation during the extraction because miRNAs
present only approximately 0.01 % of total RNA. In this
study, we used Triton X-100 (TX-100) to improve the extraction
efficiency of miRNAs with TRIzol？ reagent, which
is a commonly used commercial microRNA isolation kit.
The concentration of TX-100 and the incubation time after
the addition of TX-100 were optimized to maximize the
extraction efficiency. The extraction recovery by a combination
of TX-100 and TRIzol？ reagent was approximately 1.9-
fold greater than that by the TRIzol？ reagent alone.We have
established a very effective extraction method for the extraction
of low-abundance miRNAs in biological samples for the
determination of miRNA levels in biomaterials.
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