Sequential average segmented microscopy for high signal-to-noise ratio motion-artifact-free in vivo heart imaging
- Sequential average segmented microscopy for high signal-to-noise ratio motion-artifact-free in vivo heart imaging
- Claudio Vinegoni; 이성온; Paolo F. Feruglio; Pasquina Marzola; Matthias Nahrendorf; Ralph Weissleder
- Issue Date
- Biomedical optics express[electronic resource].
- VOL 4, NO 10, 2095-2106
- In vivo imaging is often severely compromised by cardiovascular
and respiratory motion. Highly successful motion compensation techniques
have been developed for clinical imaging (e.g. magnetic resonance
imaging) but the use of more advanced techniques for intravital microscopy
is largely unexplored. Here, we implement a sequential cardiorespiratory
gating scheme (SCG) for averaged microscopy. We show that SCG is very
efficient in eliminating motion artifacts, is highly practical, enables high
signal-to-noise ratio (SNR) in vivo imaging, and yields large field of views.
The technique is particularly useful for high-speed data acquisition or for
imaging scenarios where the fluorescence signal is not significantly above
noise or background levels.
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