Highly sensitive localized surface plasmon resonance immunosensor for label-free detection of HIV-1

Title
Highly sensitive localized surface plasmon resonance immunosensor for label-free detection of HIV-1
Authors
이진호김병찬오병근최정우
Keywords
AIDS; HIV-1 virus; Localized surface plasmon resonance; Immunosensor; Biosensor
Issue Date
2013-10
Publisher
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Citation
VOL 9, NO 7, 1018-1026
Abstract
A highly sensitive label-free immunosensor for the detection of HIV-1 is newly developed based on localized surface plasmon resonance (LSPR) method. Uniform nanopattern of circular Au-dots (10-20 nm) was fabricated on indium tin oxide (ITO) coated glass substrate by simple electrochemical deposition method. The surface of Au nanopattern was modified with HIV-1 neutralizing gp120 monoclonal antibody fragments. The modified substrate was employed to measure various concentrations of HIV-1 particles quantitatively based on the shift of longitudinal wavelength in the UV–Vis spectrum which results from the changes of local refractive index induced by specific antigen-antibody recognition events. The detection limit of the HIV-1 particles was estimated to be 200 fg/mL, which is 10 fold higher than that of previously reported virus detection method based on LSPR. Since fabricated LSPR immunosensor has high sensitivity and selectivity, it is a promising approach for biological/medical sample analysis and various kinds of virus detection.
URI
http://pubs.kist.re.kr/handle/201004/45898
ISSN
15499634
Appears in Collections:
KIST Publication > Article
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