Highly sensitive localized surface plasmon resonance immunosensor for label-free detection of HIV-1
- Highly sensitive localized surface plasmon resonance immunosensor for label-free detection of HIV-1
- 이진호; 김병찬; 오병근; 최정우
- AIDS; HIV-1 virus; Localized surface plasmon resonance; Immunosensor; Biosensor
- Issue Date
- NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
- VOL 9, NO 7, 1018-1026
- A highly sensitive label-free immunosensor for the detection of HIV-1 is newly developed based on localized surface plasmon resonance
(LSPR) method. Uniform nanopattern of circular Au-dots (10-20 nm) was fabricated on indium tin oxide (ITO) coated glass substrate by
simple electrochemical deposition method. The surface of Au nanopattern was modified with HIV-1 neutralizing gp120 monoclonal
antibody fragments. The modified substrate was employed to measure various concentrations of HIV-1 particles quantitatively based on the
shift of longitudinal wavelength in the UV–Vis spectrum which results from the changes of local refractive index induced by specific
antigen-antibody recognition events. The detection limit of the HIV-1 particles was estimated to be 200 fg/mL, which is 10 fold higher than
that of previously reported virus detection method based on LSPR. Since fabricated LSPR immunosensor has high sensitivity and selectivity,
it is a promising approach for biological/medical sample analysis and various kinds of virus detection.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.