Enhanced Production of 2,3-butanediol Using a Newly Isolated Klebsiella oxytoca M1
- Enhanced Production of 2,3-butanediol Using a Newly Isolated Klebsiella oxytoca M1
- 조숙형; 김태연; 우한민; 엄영순
- 2,3-butanediol; Klebsiella oxytoca
- Issue Date
- WCCE9 & APCChE2013
- 2,3-butanediol (2,3-BDO) production has been an increasing interest because of its wide industrial uses. In our previous study, a newly isolated Klebsiella oxytoca M1 produced 2,3-BDO as a major product and acetoin as a by-product. To optimize fermentation conditions for 2,3-BDO production by K. oxytoca M1, the effects of agitation speed and base for pH control on 2,3-BDO production were investigated. When using 5 N KOH to control pH at 6, 2,3-BDO production was higher at an agitation speed of 400 rpm than at 200 rpm (109 g/L vs. 75 g/L). Additionally, when pH was controlled using 5N NaOH, 2,3-BDO production further increased up to 129 g/L at 400 rpm, but acetoin production also increased up to 35 g/L. When using 5 N NaOH at an agitation speed of 300 rpm, a higher 2,3-BDO (152 g/L) and a lower acetoin production (20 g/L) were achieved than those at 400 rpm. To decrease acetoin production and achieve high 2,3-BDO production, the budC encoding acetoin reductase which converts acetoin to 2,3-BDO was overexpressed in K. oxytoca M1. As a result, the concentration of 2,3-BDO enhanced to 144 g/L and acetoin production decreased to 17 g/L at 400 rpm. Furthermore, the maximum 2,3-BDO titer at 300 rpm using the engineered strain was 170 g/L and acetoin production was 16 g/L. To the best of our knowledge, these results are new records on 2,3-BDO production by K. oxytoca. Further optimization and strain development would provide an improved 2,3-BDO production using K. oxytoca M1.
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