Optogenetic Stimulation in Subthalamic Nucleus influences Motor Cortex of Parkinsonian Mice

Optogenetic Stimulation in Subthalamic Nucleus influences Motor Cortex of Parkinsonian Mice
Optogenetic; Parkinson's Disease; Subthalamic Nucleus; Motor Cortex; Deep Brain Stimulation
Issue Date
Asian Pacific Conference on Biomechanics
Parkinson’s disease (PD) is one of the most common neurodegenerative disorders resulting from the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc), leading to abnormal neuronal activity in the basal ganglia (BG). On the basis of observations from the animal models of PD, high-frequency (>90 Hz) stimulation (HFS) of the subthalamic nucleus (STN) is now considered as a highly effective neurosurgical therapy for PD. However, the underlying mechanism of deep brain stimulation (DBS) still remains unclear in a matter of that electrical stimulation creates artifacts that prevent direct observation of local field potential (LFP) during DBS. Also, characteristics of firing patterns within primary motor cortex (M1) neurons need to be observed in details since M1 is directly related to pathological outputs of motor symptoms. The aim of this study was to observe responses of M1 layer V neurons in unilateral 6-hydroxydopamine (6-OHDA) lesioned Thy1::ChR2 transgenic mice model of PD during selectively stimulating of afferent axons in STN using optogenetics. The electrophysiological responses in both STN and M1 during 20 Hz low frequency optical stimulation were recorded. A synchrony of the abnormal burst firing was observed in STN and M1 when the light was off. Optical low frequency stimulation (20 Hz, 5-ms pulse width) of STN produced synchronized response of tonic firing in M1 in accordance with 20 Hz spiking in STN verifying the subthalamo-cortical circuit. As the result of this study demonstrates that the optogenetic stimulation in STN affects the LFP of M1 layer V neurons, the therapeutic relations of subthalamo-cortical neurons during high frequency DBS will be studied in the future using both optrode recordings and functional magnetic resonance imaging (fMRI).
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