A Monoclonal Antibody Against the Human SUMO-1 Protein Obtained by Immunization with Recombinant Protein and CpG-DNA-liposome Complex

Title
A Monoclonal Antibody Against the Human SUMO-1 Protein Obtained by Immunization with Recombinant Protein and CpG-DNA-liposome Complex
Authors
Dongbum Kim이주영송대근Sanghoon KwonYounghee Lee판철호Hyung-Joo Kwon
Keywords
Monoclonal antibody; SUMO-1; CpG-DNA-liposome complex
Issue Date
2013-10
Publisher
MONOCLONAL ANTIBODIES IN IMMUNODIAGNOSIS AND IMMUNOTHERAPY
Citation
VOL 32, NO 5, 354-361
Abstract
Post-translational modification regulated by conjugation of a small ubiquitin-like modifier (SUMO) is involved in various cellular processes. In this study, we expressed and purified recombinant human SUMO-1 (hSUMO-1). BALB/c mice were immunized with a complex of hSUMO-1 protein and Lipoplex(O) to produce hSUMO-1- specific antibodies. Using conventional hybridoma technology, we obtained four hybridoma clones derived from the mouse with the highest antibody titer against hSUMO-1. Based onWestern blot analysis, our hSUMO-1 monoclonal antibody specifically recognizes hSUMO-1, but not other SUMO proteins. These results support that the anti-hSUMO-1 monoclonal antibody produced with the aid of Lipoplex(O) adjuvant is specific and that Lipoplex(O) is useful for development of monoclonal antibodies against recombinant protein. In addition, we analyzed human tissues to examine the distribution of hSUMO-1. Higher expression of hSUMO-1 was detected in normal adrenal gland, esophagus, pancreas, liver, stomach, kidney, and uterus than in corresponding cancer tissues, suggesting a tumor suppressive function of hSUMO-1.
URI
http://pubs.kist.re.kr/handle/201004/46028
ISSN
21679436
Appears in Collections:
KIST Publication > Article
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