A Monoclonal Antibody Against the Human SUMO-1 Protein Obtained by Immunization with Recombinant Protein and CpG-DNA-liposome Complex
- A Monoclonal Antibody Against the Human SUMO-1 Protein Obtained by Immunization with Recombinant Protein and CpG-DNA-liposome Complex
- Dongbum Kim; 이주영; 송대근; Sanghoon Kwon; Younghee Lee; 판철호; Hyung-Joo Kwon
- Monoclonal antibody; SUMO-1; CpG-DNA-liposome complex
- Issue Date
- MONOCLONAL ANTIBODIES IN IMMUNODIAGNOSIS AND IMMUNOTHERAPY
- VOL 32, NO 5, 354-361
- Post-translational modification regulated by conjugation of a small ubiquitin-like modifier (SUMO) is involved
in various cellular processes. In this study, we expressed and purified recombinant human SUMO-1 (hSUMO-1).
BALB/c mice were immunized with a complex of hSUMO-1 protein and Lipoplex(O) to produce hSUMO-1-
specific antibodies. Using conventional hybridoma technology, we obtained four hybridoma clones derived
from the mouse with the highest antibody titer against hSUMO-1. Based onWestern blot analysis, our hSUMO-1
monoclonal antibody specifically recognizes hSUMO-1, but not other SUMO proteins. These results support that
the anti-hSUMO-1 monoclonal antibody produced with the aid of Lipoplex(O) adjuvant is specific and that
Lipoplex(O) is useful for development of monoclonal antibodies against recombinant protein. In addition, we
analyzed human tissues to examine the distribution of hSUMO-1. Higher expression of hSUMO-1 was detected
in normal adrenal gland, esophagus, pancreas, liver, stomach, kidney, and uterus than in corresponding cancer
tissues, suggesting a tumor suppressive function of hSUMO-1.
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