Effect of Formic Acid on Butanol Production from Glucose and Glycerol by Clostridium pasteurianum DSM 525
- Effect of Formic Acid on Butanol Production from Glucose and Glycerol by Clostridium pasteurianum DSM 525
- 김태연; 우한민; 서진호; 엄영순
- Formic acid; Clostridium; Butanol
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- Formate was reported to prevent the switch of acidogenesis to solventogenesis in the fermentation by Clostridium acetobutylicum . But the solventogenesis of Clostridium beijerinkii was not affected by formate addition . Here, we tested whether the formate addition could change the fermentation profile of Clostridium pasteurianum DSM 525. C. pasteurianum undergo acidogenic or solventogenic pathway depending on carbon source and the main product is butyric acid from glucose and butanol from glycerol. The fermentation was individually performed with glucose or glycerol as a sole carbon source (each 20 g/L) in the presence of 1 g/L formate. However, formate had no effect on final product distribution or carbon consumption rates at both glucose and glycerol fermentation compared with controls (no formate addition). Moreover, the final concentration of butyrate (in glucose fermentation) and butanol (in glycerol fermentation) was almost the same with that of control. Consequently, the physiological role of formic acid in C. pasteurianum is similar with in C. beijerinkii rather in C. acetobutylicum.
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