Stringent oxidative and stress responses of Corynebacterium glutamicum for furfural, a hydrolysate inhibitor revealed by genome-wide transcriptome analysis
- Stringent oxidative and stress responses of Corynebacterium glutamicum for furfural, a hydrolysate inhibitor revealed by genome-wide transcriptome analysis
- 박홍실; 엄영순; 심상준; 우한민
- Hydrolysate inhibitor; Corynebacterium glutamicum
- Issue Date
- 2013 한국생물공학회 추계학술발표대회 및 국제심포지엄
- Pretreatment of lignocellulosic biomasses by acid hydrolysis generates cellular inhibitors such as Furfural, HMF, etc. Therefore, it is necessary to overcome the toxicity in the hydrolysate. We focused the effect of the inhibitors to Corynebacterium glutamicum, which is widely used for industrial strains. Transcriptome analysis of C. glutamicum grown in the presence of furfural was investigated; resulting in expression of many genes fluctuated, compared to wild type. Especially, SOS response genes were markedly up-regulated and some of these genes seemed to be related with oxidative stress. Indeed, C. glutamicum grown with furfural showed over 2-folds of reactive oxygen species stresses. This could be useful for metabolic engineering of furfural tolerant C. glutamicum. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (2013, University-Institute cooperation program) and ‘Creative Allied Program (CAP)’ through the Korea Research Council of Fundamental Science and Technology (KRCF) and Korea Institute of Science and Technology (KIST).
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- KIST Publication > Conference Paper
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