High production of 2,3-butanediol from glycerol using engineered Klebsiella oxytoca M1 by reducing by-product formation
- High production of 2,3-butanediol from glycerol using engineered Klebsiella oxytoca M1 by reducing by-product formation
- 조숙형; 우한민; 엄영순
- 2,3-BDO; glycerol; Klebsiella oxytoca
- Issue Date
- 2013 한국생물공학회 추계학술발표대회 및 국제심포지엄
- Glycerol is an attractive feedstock for biofuel/chemical production because it is an inexpensive compound generated as a by-product from biodiesel process. In addition, the highly reduced form of glycerol provides distinct advantages over other carbohydrates when one considers the production of reduced chemicals. In this study, we report the high production of 2,3-butanediol (2,3-BDO) from glycerol using a newly isolated Klebsiella oxytoca M1. Glycerol was converted to 2,3-BDO (13.9 g/L) by K. oxytoca M1, but 1,3-PDO was produced mainly over 30 g/L in fed-batch fermentation at pH 6. By deleting the dhaB gene encoding glycerol dehydratase in 1,3-PD synthesis pathway, 1,3-PDO production was remarkably reduced below 1 g/L and 2,3-BDO production was increased (24.36 g/L), whereas lactic acid was produced up to 29.8 g/L. To enhance the carbon flux to 2,3-BDO, the engineered strain deficient in 1,3-PDO and lactic acid formation was successfully constructed by deleting both the dhaB and the ldhA (encoding lactate dehydrogenase). As a result, 2,3-BDO concentration, yield, productivity were significantly improved by using K. oxytoca M1 ∆dhaB ∆ldhA, reaching 115 g/L, 0.45 g/g, 1.01 g/L∙H, respectively. To the best of our knowledge, this result is the highest 2,3-BDO production from glycerol by K. oxytoca.
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