Enhanced xylose utilization by Bacillus sp. adapted on xylose and 2-deoxy-D-glucose
- Enhanced xylose utilization by Bacillus sp. adapted on xylose and 2-deoxy-D-glucose
- 강금래; 김기연; 김은숙; 우한민; 김경헌; 엄영순
- bacillus; xylose; adaptation
- Issue Date
- 2013 한국생물공학회 추계학술발표대회 및 국제심포지엄
- Bacillus species are one of the Generally Recognized As Safe (GRAS) used for producing acetoin in industrial field. Acetoin is an important biorefinery platform chemical used in not only dairy products but also chemical synthesis and cosmetics. In previous studies, it was reported that Bacillus was able to utilize various carbon sources. However, they cannot effectively utilize xylose, which is abundant in cellulosic biomass because there is no specific xylose transporter. To overcome the problem of xylose utilization, we developed an adapted Bacillus strain using a newly isolated SPF35 from soil of Indonesia. SPF35 produced acetoin as a main product in glucose, cellobiose and xylose medium, but could not utilize xylose efficiently. Adapted SPF35-1 was developed to improve xylose utilization through serial transfer of SPF35 in xylose with a 2-deoxy-D-glucose (2DG). The final adapted SPF35-1 could consume 10 g/L xylose completely whereas the control SPF35 consumed only 3.14 g/L xylose in 24h. Further optimization and strain development would enable SPF35 to produce acetoin by cofermentation of glucose and xylose.
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