Enhanced expression of an endoglucanase in Bacillus sp. SPF35 by using two tandem promoters
- Enhanced expression of an endoglucanase in Bacillus sp. SPF35 by using two tandem promoters
- 김은숙; 우한민; 엄영순
- cellulose; cellulase; bacillus; overexpression
- Issue Date
- 2013년 한국미생물학회연합 국제학술대회
- Cellulosic biomass is the most abundant renewable bioresource for the production of bio-based products and bioenergy. Recent studies focus on hydrolyzing cellulosic biomass more economically for biorefinery process and consolidated bioprocessing (CBP) by cellulolytic microorganisms has been proposed. Recently, cellulolytic Bacillus SPF35 was isolated from soil of Indonesia. In a previous study, it was reported that overexpression of B. subtilis endoglucanase enabled to grow on cellulosic medium. To enhance an expression level of endoglucanase in SPF35, strong promoter was screened using GFP gene as a reporter gene and intensity of two promoters arranged in tandem was analyzed. Among the tandem promoters, Pspac/P43 tandem promoters could lead to 2-fold higher level of fluorescence activity compared to the strongest single promoter P43. Application of the tandem promoter in endoglucanase production resulted in 2-fold bigger halo zone on cellulose plate and growth (CFU/ml) was 25-fold higher than wild type strain on cellulose medium. These tandem promoters were shown to be functional in expression of SPF35 endoglucanase and have an application potential in CBP by cellulolytic microorganisms.
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