Influence of additional CO₂ supply on cyanobacteria, a photosynthetic platform of direct CO₂ conversion to biochemicals
- Influence of additional CO₂ supply on cyanobacteria, a photosynthetic platform of direct CO₂ conversion to biochemicals
- 좌준원; 강민경; 엄영순; 심상준; 우한민
- Cyanobacteria; CO₂conversion; Biochemical; Environment
- Issue Date
- 2013 한국생물공학회 추계학술발표대회 및 국제심포지엄
- Due to uprising global warming and understanding of positive carbon cycle, a photosynthetic cyanobacterium has gather attentions to capture solar energy and convert CO2 to various metabolites. Low solubility of CO2 in culture medium is a major concern to maximize product yield using cyanobacteria. Here we investigate the effect of additional CO2 supply in the medium on the cellular growth of both wild type and mutant. We used newly designed Erlenmeyer flasks with three ports and gas mixer system with continuous 5% CO2 into the BG-11 medium. Also, we added MOPS buffer to stabilize the pH of the medium to prevent the lower cellular growth from the pH decrease at early growth phase, which might be crucial for the cellular growth. As a result, the cellular growth of cyanobacteria under 5% CO2 Supply showed higher optical density, compared to the wild type. Moreover, Synechococcus elongatus PCC7942 harboring the green fluorescent gene on chromosome using SynBrick vectors was constructed and the influence of additional CO2 supply under modified BG-11 on the mutant was investigated. This culture system would help to increase the yield of biochemical production from direct CO2 using an engineered cyanobacteria. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (2013, University-Institute cooperation program) and ‘Creative Allied Program (CAP)’ through the Korea Research Council of Fundamental Science and Technology (KRCF) and Korea Institute of Science and Technology (KIST).
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