Magnetic-bead-based immunoassay using E. coli cells with autodisplayed Z-domains

Title
Magnetic-bead-based immunoassay using E. coli cells with autodisplayed Z-domains
Authors
유구봉지홍박민강민정Joachim Jose변재철
Keywords
Magnetic bead; E. coli; Autodisplay; Z-domain; Immunoassay; Surface modification
Issue Date
2013-07
Publisher
Enzyme and microbial technology
Citation
VOL 53, NO 2, 118-122
Abstract
Escherichia coli cells with autodisplayed Z-domains could increase the sensitivity of immunoassays by immobilizing antibodies in a controlled orientation. In the work presented here, E. coli cells with autodisplayed Z-domains were immobilized to magnetic beads for subsequent immunoassay. In comparing conventional immunoassay using the E. coli cells with autodisplayed Z-domains, the magnetic-beadbased immunoassay improved immunoassay efficiency by minimizing the loss of E. coli cells during repeated centrifugation steps during washing. For the immobilization of E. coli cells to magnetic beads, the magnetic beads were modified with poly-l-lysine to bind to negatively charged E. coli cells. During the surface modification process, physical parameters such as the surface charge and size of the magnetic beads were analyzed to confirm the formation of E. coli-magnetic bead complexes. To test the feasibility of the magnetic-bead-based immunoassay, horseradish peroxidase (HRP) was used as a model analyte, and a biomarker for inflammatory diseases, C-reactive protein (CRP), was used for a demonstration of an application in medical diagnosis.
URI
http://pubs.kist.re.kr/handle/201004/46065
ISSN
01410229
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KIST Publication > Article
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