Flow cytometric immunoassay using E. coli with autodisplayed Z-domains

Title
Flow cytometric immunoassay using E. coli with autodisplayed Z-domains
Authors
박민봉지홍유구Joachim Jose강민정변재철
Keywords
Flow cytometry; Autodisplay; Z-domain; Immunoassay; CRP; Hepatitis B virus surface antigen; C-reactive protein
Issue Date
2013-08
Publisher
Enzyme and microbial technology
Citation
VOL 53, NO 3, 181-188
Abstract
Recently, we reported a highly sensitive immunoassay using Escherichia coli cells with autodisplayed Z-domains. In this work, E. coli cells with autodisplayed Z-domains were applied to the flow-cytometrybased simultaneous detection of multiple analytes. The E. coli cells were doubly transfected to express a fluorescent protein (tdTomato) in the cytosol and the autodisplayed Z-domains on the outer membrane. By using E. coli cells with only the autodisplayed Z-domains, immunoassay of multiple analytes could be performed simultaneously on the same sample. Flow cytometry can be used to identify the immunoassay type by simultaneously detecting the fluorescence signal from the cytosol (tdTomato) and the fluorescence from the outer membrane, enabling the quantification of bound analytes after treatment with additional fluorescently labeled antibodies. To demonstrate the immunoassay of multiple analytes by using flow cytometry, human hepatitis B virus surface antigen (HBsAg) and C-reactive protein (CRP), a broad spectrum inflammation marker, were used as model analytes.
URI
http://pubs.kist.re.kr/handle/201004/46070
ISSN
01410229
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KIST Publication > Article
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