Full metadata record

DC FieldValueLanguage
dc.contributor.authorJoshua S. Grimley-
dc.contributor.authorLi Li-
dc.contributor.authorWeina Wang-
dc.contributor.author레이웬-
dc.contributor.authorLorena S. Beese-
dc.contributor.authorHommeW. Hellinga-
dc.contributor.author조지어거스틴-
dc.date.accessioned2015-12-03T01:02:43Z-
dc.date.available2015-12-03T01:02:43Z-
dc.date.issued201310-
dc.identifier.citationVOL 33, NO 41, 16297-16309-
dc.identifier.issn02706474-
dc.identifier.other40510-
dc.identifier.urihttp://pubs.kist.re.kr/handle/201004/46154-
dc.description.abstractWe describe an engineered fluorescent optogenetic sensor, SuperClomeleon, that robustly detects inhibitory synaptic activity in single, cultured mouse neurons by reporting intracellular chloride changes produced by exogenous GABA or inhibitory synaptic activity. Using a cell-free protein engineering automation methodology that bypasses gene cloning, we iteratively constructed, produced, and assayed hundreds of mutations in binding-site residues to identify improvements in Clomeleon, a first-generation, suboptimal sensor. Structural analysis revealed that these improvements involve halide contacts and distant side chain rearrangements. The development of optogenetic sensors that respond to neural activity enables cellular tracking of neural activity using optical, rather than electrophysiological, signals. Construction of such sensors using in vitro protein engineering establishes a powerful approach for developing new probes for brain imaging.-
dc.publisherJournal of neuroscience-
dc.titleVisualization of Synaptic Inhibition with an Optogenetic Sensor Developed by Cell-Free Protein Engineering Automation-
dc.typeArticle-
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE