Determination of major phlorotannins in Eisenia bicyclis using hydrophilic interaction chromatography: Seasonal variation and extraction characteristics
- Determination of major phlorotannins in Eisenia bicyclis using hydrophilic interaction chromatography: Seasonal variation and extraction characteristics
- 김상민; 강석우; 전제승; 정유진; 김우리; 김철영; 엄병헌
- Eisenia bicyclis; Hydrophilic interaction chromatography; nutraceutical; Pharmaceutical; Phlorotannins; Seasonal variation
- Issue Date
- Food chemistry
- VOL 138, NO 4, 2399-2406
- In this study, a hydrophilic interaction chromatography (HILIC) condition was developed for the
simultaneous determination of five major phlorotannins from an extract of Eisenia bicyclis (Kjellman)
Setchell with good linearity (r2 > 0.999). Based on this method, the seasonal variations and extraction
characteristics, in terms of total extraction yield and the content of the phlorotannins, were investigated
under various extraction conditions. In results, the yields and phlorotannins were increased two-to-four
times in summer (June–October) and then, were decreased to normal levels in winter (November–March).
In the extraction of E. bicyclis, ethanol percentage in water, extraction time and washing time significantly
affected the yield of the extract and the phlorotannins, whereas the temperature and the sample/solvent
ratio impacted the extraction to a lesser degree. These results will be useful information in the application
of this macroalga in the commercial areas related to nutraceuticals, pharmaceuticals, and cosmeceuticals.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.