Microfluidic based biosensing for Escherichia coli detection by embedding antimicrobial peptide-labeled beads
- Microfluidic based biosensing for Escherichia coli detection by embedding antimicrobial peptide-labeled beads
- 유정하; 우덕하; 장미숙; 전명석
- Antimicrobial peptide; Biosensor; Detection efficiency; Escherichia coli; Microbead; Microfluidic chip
- Issue Date
- Sensors and actuators. B, Chemical
- VOL 191, 211-218
- Due to their ability of effective binding to multiple target microbes, the antimicrobial peptides (AMPs)have recently received lots of attention as an alternative to antibodies for detecting bacteria. We devel-oped a new biosensing method to detect Escherichia coli (E. coli) by implementing a microfluidic chipdesigned with a weir inside the channel, in which AMP-labeled microbeads were embedded. We char-acterized the detection rate of the stained E. coli within a certain period of time to examine the detectioneffectiveness of our device. As the flow rate of the bacterial suspension increases, the detection time toreach the saturation level decreases to less than 30 min, suggesting rapid detection, while the detectionefficiency is maintained at a similar level. Except with very low concentrations of E. coli (<103 cells/mL),both the detection time and the efficiency do not depend on the E. coli concentration. Our method has thepotential to be developed as a novel biosensing platform for rapid and accurate detection of pathogens.
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