Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo
- Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo
- Darian D. Cash; Osnat Cohen-Zontag; 김낙균; Kinneret Shefer; Yogev Brown; Nikolai B. Ulyanov; Yehuda Tzfati; Juli Feigon
- RNA triplex; yeast; RNA structure; Hoogsteen
- Issue Date
- Proceedings of the National Academy of Sciences of the United States of America
- VOL 110, NO 27, 10970-10975
- Telomerase is a ribonucleoprotein complex that extends the 3′ ends
of linear chromosomes. The specialized telomerase reverse transcriptase
requires a multidomain RNA (telomerase RNA, TER), which
includes an integral RNA template and functionally important template-
adjacent pseudoknot. The structure of the human TER pseudoknot
revealed that the loops interact with the stems to form
a triple helix shown to be important for activity in vitro. A similar
triple helix has been predicted to form in diverse fungi TER pseudoknots.
The solution NMR structure of the Kluyveromyces lactis
pseudoknot, presented here, reveals that it contains a long pyrimidine
motif triple helix with unexpected features that include three
individual bulge nucleotides and a C+•G-C triple adjacent to a stem
2–loop 2 junction. Despite significant differences in sequence and
base triples, the 3D shape of the human and K. lactis TER pseudoknots
are remarkably similar. Analysis of the effects of nucleotide
substitutions on cell growth and telomere lengths provides evidence
that this conserved structure forms in endogenously assembled
telomerase and is essential for telomerase function in vivo.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.