Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo

Title
Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo
Authors
Darian D. CashOsnat Cohen-Zontag김낙균Kinneret SheferYogev BrownNikolai B. UlyanovYehuda TzfatiJuli Feigon
Keywords
RNA triplex; yeast; RNA structure; Hoogsteen
Issue Date
2013-07
Publisher
Proceedings of the National Academy of Sciences of the United States of America
Citation
VOL 110, NO 27, 10970-10975
Abstract
Telomerase is a ribonucleoprotein complex that extends the 3′ ends of linear chromosomes. The specialized telomerase reverse transcriptase requires a multidomain RNA (telomerase RNA, TER), which includes an integral RNA template and functionally important template- adjacent pseudoknot. The structure of the human TER pseudoknot revealed that the loops interact with the stems to form a triple helix shown to be important for activity in vitro. A similar triple helix has been predicted to form in diverse fungi TER pseudoknots. The solution NMR structure of the Kluyveromyces lactis pseudoknot, presented here, reveals that it contains a long pyrimidine motif triple helix with unexpected features that include three individual bulge nucleotides and a C+•G-C triple adjacent to a stem 2–loop 2 junction. Despite significant differences in sequence and base triples, the 3D shape of the human and K. lactis TER pseudoknots are remarkably similar. Analysis of the effects of nucleotide substitutions on cell growth and telomere lengths provides evidence that this conserved structure forms in endogenously assembled telomerase and is essential for telomerase function in vivo.
URI
http://pubs.kist.re.kr/handle/201004/46241
ISSN
00278424
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KIST Publication > Article
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