A dynamically cultured collagen/cells-incorporated elastic scaffold for small-diameter vascular grafts

Title
A dynamically cultured collagen/cells-incorporated elastic scaffold for small-diameter vascular grafts
Authors
박인수김영하정영미김수현김상헌
Keywords
tissue engineeing; blood vessel; scaffold; vascular graft; Small-diameter vascular grafts; smooth muscle cells; endothelial cells; collagen gel; biodegradable elastic scaffold; bioreactor
Issue Date
2012-12
Publisher
Journal of biomaterials science, Polymer edition
Citation
VOL 23, NO 14, 1807-1820
Abstract
There is an essential demand for tissue-engineered autologous small-diameter vascular grafts, which offer temporary supports and guides for vascular tissue organization, repair and remodeling. This study reports on the effect of collagen/smooth muscle cells (SMCs) mixtures under dynamic cultures and SMC–endothelial cell (ECs) co-culture on cell proliferation, uniform cell distribution, extracellular matrix deposition, and endothelial cells monolayer formation in tissue-engineered tubular arterial constructs of 4 mm inner diameter. Rabbit aortic SMCs were infiltrated with collagen solution in poly(L-lactide-co-ε-caprolactone) (PLCL) scaffolds under vacuum to form collagenous gel and subjected to dynamic strain by culturing them in a dynamic perfusion bioreactor. The construct lumen was subsequently seeded with ECs and experiments were completed to create ECs–SMCs co-culture constructs. The collagen/SMCs incorporated elastic scaffold cultured under dynamic culture conditions promoted matrix deposition, leading to the development of tissue-engineered vascular constructs, and induced SMC to have more uniform cell distribution. Scanning electron microscopic examination and von Willebrand Factor staining demonstrated the presence of ECs spread over the lumen. Quantitative analysis of elastin contents demonstrated that the engineered vessels acquired similar elastin contents as native arteries. The collagen/SMCs/ECs incorporated PLCL scaffolds under dynamic culture conditions can be used as a scaffold for tissue engineering to facilitate small-diameter vascular-tissue formation.
URI
http://pubs.kist.re.kr/handle/201004/46290
ISSN
09205063
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KIST Publication > Article
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