In Situ Biphasic Extractive Fermentation for Hexanoic Acid Production from Sucrose by Megasphaera elsdenii NCIMB 702410

Title
In Situ Biphasic Extractive Fermentation for Hexanoic Acid Production from Sucrose by Megasphaera elsdenii NCIMB 702410
Authors
최기은전병승김병천오민규엄영순상병인
Keywords
hexanoic acid; fermentation; sucrose; Megasphaera; Alamine 336; In situ biphasic extraction; Megasphaera elsdenii NCIMB 702410; Batch culture
Issue Date
2013-11
Publisher
Applied biochemistry and biotechnology
Citation
VOL 171, NO 5, 1094-1107
Abstract
Hexanoic acid production by a bacterium using sucrose as an economic carbon source was studied under conditions in which hexanoic acid was continuously extracted by liquid–liquid extraction. Megasphaera elsdenii NCIMB 702410, selected from five M. elsdenii strains, produced 4.69 g l−1 hexanoic acid in a basal medium containing sucrose. Production increased to 8.19 g l−1 when the medium was supplemented by 5 g l−1 sodium butyrate. A biphasic liquid–liquid extraction system with 10 % (v/v) alamine 336 in oleyl alcohol as a solvent was evaluated in a continuous stirred-tank reactor held at pH 6. Over 90%(w/w) of the hexanoic acid in a 0.5M aqueous solution was transferred to the extraction solvent within 10 h. Cell growth was not significantly inhibited by direct contact of the fermentation broth with the extraction solvent. The system produced 28.42 g l−1 of hexanoic acid from 54.85 g l−1 of sucrose during 144 h of culture, and 26.52 and 1.90 g l−1 of hexanoic acid was accumulated in the extraction solvent and the aqueous fermentation broth, respectively. The productivity and yield of hexanoic acid were 0.20 g l−1 h−1 and 0.50 g g−1 sucrose, respectively.
URI
http://pubs.kist.re.kr/handle/201004/46324
ISSN
02732289
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KIST Publication > Article
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