Real-Time Observation of Multiple-Protein Complex Formation with Single-Molecule FRET

Title
Real-Time Observation of Multiple-Protein Complex Formation with Single-Molecule FRET
Authors
배우리최말기현창봉신년균윤태영
Issue Date
2013-06
Publisher
Journal of the American Chemical Society
Citation
VOL 135, NO 28, 10254-10257
Abstract
Current single-molecule techniques do not permit the real-time observation of multiple proteins interacting closely with each other. We here report an approach enabling us to determine the single-molecule fluorescence resonance energy transfer (FRET) kinetics of multiple protein–protein interactions occurring far below the diffraction limit. We observe a strongly cooperative formation of multimeric soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, which suggests that formation of the first SNARE complex triggers a cascade of SNARE complex formation.
URI
http://pubs.kist.re.kr/handle/201004/46358
ISSN
00027863
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KIST Publication > Article
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