Real-Time Observation of Multiple-Protein Complex Formation with Single-Molecule FRET
- Real-Time Observation of Multiple-Protein Complex Formation with Single-Molecule FRET
- 배우리; 최말기; 현창봉; 신년균; 윤태영
- Issue Date
- Journal of the American Chemical Society
- VOL 135, NO 28, 10254-10257
- Current single-molecule techniques do not permit the real-time observation of multiple proteins interacting closely with each other. We here report an approach enabling us to determine the single-molecule fluorescence resonance energy transfer (FRET) kinetics of multiple protein–protein interactions occurring far below the diffraction limit. We observe a strongly cooperative formation of multimeric soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, which suggests that formation of the first SNARE complex triggers a cascade of SNARE complex formation.
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