Mechanical unzipping and rezipping of a single SNARE complex reveals hysteresis as a force-generating mechanism

Title
Mechanical unzipping and rezipping of a single SNARE complex reveals hysteresis as a force-generating mechanism
Authors
민두영김기범현창봉조용훈신년균윤태영
Keywords
Biological sciences; Biophysics; Cell biology
Issue Date
2013-04
Publisher
Nature Communications
Citation
VOL 4, 1705-1-1705-10
Abstract
Formation of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex provides mechanical thrust for membrane fusion, but its molecular mechanism is still unclear. Here using magnetic tweezers, we observe mechanical responses of a single neuronal SNARE complex under constant pulling force. Single SNARE complexes may be unzipped with 34 pN force. When rezipping is induced by lowering the force to 11 pN, only a partially assembled state results, with the C-terminal half of the SNARE complex remaining disassembled. Reassembly of the C-terminal half occurs only when the force is further lowered below 11 pN. Thus, mechanical hysteresis, characterized by the unzipping and rezipping cycle of a single SNARE complex, produces the partially assembled state. In this metastable state, unzipping toward the N-terminus is suppressed while zippering toward the C-terminus is initiated as a steep function of force. This ensures the directionality of SNARE-complex formation, making the SNARE complex a robust force-generating machine.
URI
http://pubs.kist.re.kr/handle/201004/46361
ISSN
20411723
Appears in Collections:
KIST Publication > Article
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