Identification of macrophage genes responsive to extracellular acidification

Title
Identification of macrophage genes responsive to extracellular acidification
Authors
Seung-Yoon Park김인산
Keywords
Gene expression; Extracellular acidi?cation; Macrophages; Microarray
Issue Date
2013-04
Publisher
Inflammation research : official journal of the European Histamine Research Society ... [et al.]
Citation
VOL 62, NO 4, 399-406
Abstract
Objective A low pH microenvironment is a characteristic feature of inflammation loci and affects the functions of immune cells. In this study, we investigated the effect of extracellular acidification on macrophage gene expression. Methods RAW264.7 macrophages were incubated in neutral (pH 7.4) or acidic (pH 6.8) medium for 4 h. Global mRNA expression levels were determined using Affymetrix genechips. Results The mRNA expressions of 353 macrophage genes were significantly modified after incubation in acidic medium; 193 were up-regulated and 160 down-regulated. Differentially regulated genes were grouped into 13 classes based on the functions of the corresponding protein products. Pathway analysis revealed that differentially expressed genes are enriched in pathways related to inflammation and immune responses. Quantitative real-time PCR analysis confirmed that the expressions of CXCL10, CXCL14, IL-18, IL-4RA, ABCA1, CCL4, IL-7R, CXCR4, TLR7, and CCL3 mRNAs were regulated by extracellular acidification. Conclusion The results of this study provide insights into the effects of acidic extracellular environments on macrophage gene expression.
URI
http://pubs.kist.re.kr/handle/201004/46404
ISSN
10233830
Appears in Collections:
KIST Publication > Article
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