The Effect of N/P Ratio on the In Vitro and In Vivo Interaction Properties of PEGylated Poly[2-(dimethylamino)ethyl methacrylate]-Based siRNA Complexes

Title
The Effect of N/P Ratio on the In Vitro and In Vivo Interaction Properties of PEGylated Poly[2-(dimethylamino)ethyl methacrylate]-Based siRNA Complexes
Authors
Dana J. GaryJungbin MinYoungwook KimKeunchil Park원유연
Keywords
biodistribution; blood toxicity; N/P ratio; polyplexes; siRNA
Issue Date
2013-08
Publisher
Macromolecular bioscience
Citation
VOL 13, NO 8, 1059-1071
Abstract
PEG-PnBA-PDMAEMA triblock and PEG-PDMAEMA diblock copolymers are used as model systems for studying the role of N/P ratio on the in vivo behavior of PEGylated siRNA carriers in mice. The presence of a free/uncomplexed polymer population coexisting with siRNA complexes is established. A change in the N/P ratio exerts no significant influence on the in vivo biodistribution and ex vivo blood chemistry properties of the respective systems. Histological analysis of major organs indicates that the presence of uncomplexed polymer elicits toxicity to the organ that is associated with the clearance of the siRNA complexes from the circulation system. This effect can be eliminated by working at N/P ratios near the charge-neutralization point of the complexes
URI
http://pubs.kist.re.kr/handle/201004/46434
ISSN
16165187
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KIST Publication > Article
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