Gene analysis of brain in aged-experimental autoimmune encephalomyelitis mice reveals over-expression of stress response pathway-related genes
- Gene analysis of brain in aged-experimental autoimmune encephalomyelitis mice reveals over-expression of stress response pathway-related genes
- 서지은; 마흐법하산; Sailendra Sarma; 강민정; 정병화; 조미라; 남석우; 박원상; 김호연; 권오승
- Issue Date
- The journal of immunology : official journal of the American Association of Immunologists
- Experimental autoimmune encephalomyelitis (EAE) is the most common animal model of multiple sclerosis. In our previous works, two different age groups of mice (6 weeks; Y vs. 15 months; O) were used to compare the severity of EAE. Significant decreases of body weight and increases of clinical score were observed in O-EAE, indicating a more severe form of disease was induced at old age. With the purpose to identify genes that contribute to this pathology, we investigated gene expressions in brain using gene microarray. As a result, 32 genes including MHC class II molecules antigen, complement component and lysozyme in Y-EAE, and 4 genes including circulatory system, cell adhesion and migration functions in O-EAE were up-regulated compared to their controls. Expression of 44 genes was significantly different between O-EAE and Y-EAE. O-EAE showed 27 up-regulated genes with related pathways including response to stress and acute inflammatory responses, and 17 down-regulated genes with related pathways including immune development, hemopoiesis and homeostatic process, compared to Y-EAE. Over-expression of the stress response pathway-related genes in O-EAE such as argine vasopressin, cyclin-dependent kinase inhibitor 1A and hypoxia-induced factor 3α were validated by qRT-PCR. Our data suggest that the highly expressed genes related to stress responses in O-EAE affected serious disease process, and these genes may be prior targets for susceptibility of EAE.
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