Electrochemical ELISA based on Escherichia coli with autodisplayed Z-domains

Title
Electrochemical ELISA based on Escherichia coli with autodisplayed Z-domains
Authors
이주경유구박민Joachim Jose강민정변재철
Keywords
Autodisplay; Z-domain; E. Coli detection; Electrochemical ELISA; Escherichia coli; Poly-L-lysine; Parylene
Issue Date
2012-12
Publisher
Sensors and actuators. B, Chemical
Citation
VOL 175, 46-52
Abstract
Recently, we reported autodisplayed Z-domains on Escherichia coli can highly improve the sensitivity of immunoassays through the orientation control of detection antibodies. In this work, the amperometric analysis was applied to the immunoassay based on E. coli cells with autodisplayed Z-domains. For amperometric analysis, the reduction current of 3,5,3′,5′-tetramethylbenzidine (TMB) was measured at the potential of −50 mV and the correlation between the reduction current and the OD value was estimated to be 28 nA/OD and the limit of detection was calculated to be 0.07 in OD unit. In this work, the feasibility of the immunoassay based on amperometric analysis was presented by the quantification of HRP as a model analyte. For the demonstration of medical diagnosis, C-reactive protein (CRP) was detected by using electrochemical ELISA based on E. coli with autodisplayed Z-domains.
URI
http://pubs.kist.re.kr/handle/201004/46530
ISSN
09254005
Appears in Collections:
KIST Publication > Article
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