Tuning Solid-State Fluorescence to the Near-Infrared: A Combinatorial Approach to Discovering Molecular Nanoprobes for Biomedical Imaging

Title
Tuning Solid-State Fluorescence to the Near-Infrared: A Combinatorial Approach to Discovering Molecular Nanoprobes for Biomedical Imaging
Authors
아제이싱임창근이용덕맹준호이상엽고준석김세훈
Keywords
aggregation-enhanced fluorescence; biomedical in vivo imaging; combinatorial chemistry; dye-concentrated nanoparticles; solid-state NIR fluorescence
Issue Date
2013-06
Publisher
ACS Applied Materials & Interfaces
Citation
VOL 5, NO 18, 8881-8888
Abstract
Dyes showing solid-state fluorescence (SSF) are intriguing molecules that can emit bright fluorescence in the condensed phase. Because they do not suffer from self-quenching of fluorescence, nanoscopic dense integration of those molecules produces particulate nanoprobes whose emission intensity can be boosted by raising the intraparticle dye density. In spite of the potential promise for imaging applications demanding intense emission signals, their excitation and emission spectra are generally limited to the visible region where biological tissues have less transparency. Therefore, the SSF-based nanoprobes have rarely been applied to noninvasive in vivo imaging. Here we report a combinatorial chemistry approach to attain a high level of tissue transparency of SSF by tuning its excitation and emission wavelengths to the truly near-infrared (NIR) region. We built a dipolar arylvinyl (ArV) scaffold-based chemical library where the optical bandgap could be narrowed to the NIR above 700 nm by combinatorial modulation of the π-electron push-pull strengths. The ArV-aggregated nanoparticles (FArV NPs) with a colloidal size less than 20 nm were formulated using a polymeric surfactant (Pluronic F-127) and applied to bioimaging in cells and in vivo. We demonstrate that some of FArV NPs have truly NIR excitation and emission of SSF, capable of noninvasive in vivo imaging (efficient lymph node mapping and early diagnosis of tumor) in mouse models by virtue of bright solid-state NIR fluorescence and high signal-to-background contrast (S/B ≈ 8) as well as facile circulation in the living body.
URI
http://pubs.kist.re.kr/handle/201004/46595
ISSN
19448244
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KIST Publication > Article
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