Quantification of antioxidant compounds from colored potatoes, 'Hongyoung' and 'Jayoung' cultivars and their protective activity on human hepatoma HepG2 cell
- Quantification of antioxidant compounds from colored potatoes, 'Hongyoung' and 'Jayoung' cultivars and their protective activity on human hepatoma HepG2 cell
- 김상민; 강경수; 하인수; 노주원; 남정환; 조지홍; 권오근; 정진철; 이재권; 판철호
- colored potato; hepatoprotective activity; anthocyanin; chlorogenic acid; antioxidant compound
- Issue Date
- VOL 29, NO 1, 45-58
- The colored Potatoes, ‘Hongyoung’ and ‘Jayoung’ cultivars bred in Rural Development Administration (Korea) contain antioxidant compounds such as chlorogenic acids and anthocyanin compounds in the tuber. In this study, main antioxidant compounds in both cultivars were identified by LC-MS analysis and quantified by HPLC analysis. The result showed that three compounds (petunidin-3-O-coumaroyl-rutinoside-5-O-glucoside, pelargonidin-3-Ocoumaroyl- rutinoside-5-O-glucoside and peonidin-3-O-coumaroyl-rutinoside -5-O-glucoside) were identified as main anthocyanins in ‘Jayoung’ cultivar while only pelargonidin-3-O-coumaroyl-rutinoside-5-O-glucoside was presented as a major anthocyanin in ‘Hongyoung’ cultivar. Among chlorogenic acids, 5-O-caffeoylquinic acid was the most abundant in both cultivars. In order to evaluate hepatoprotective activity of these potatoes, the methanol extracts and their serial solvent fractions were prepared by solvent partition and XAD-7 column chromatography method and treated on hepatoma HepG2 cells of which cytotoxicity was induced by tert-butyl hydroperoxide. Among the tested samples, anthocyanin-rich fractions from both cultivars contained the strongest hepatoprotective effect on HepG2 cells. This result indicates that hepatoprotective effect of these fractions can be occurred from anthocyanin.
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