Quantification of antioxidant compounds from colored potatoes, 'Hongyoung' and 'Jayoung' cultivars and their protective activity on human hepatoma HepG2 cell

Title
Quantification of antioxidant compounds from colored potatoes, 'Hongyoung' and 'Jayoung' cultivars and their protective activity on human hepatoma HepG2 cell
Authors
김상민강경수하인수노주원남정환조지홍권오근정진철이재권판철호
Keywords
colored potato; hepatoprotective activity; anthocyanin; chlorogenic acid; antioxidant compound
Issue Date
2013-11
Publisher
과학교육연구논총
Citation
VOL 29, NO 1, 45-58
Abstract
The colored Potatoes, ‘Hongyoung’ and ‘Jayoung’ cultivars bred in Rural Development Administration (Korea) contain antioxidant compounds such as chlorogenic acids and anthocyanin compounds in the tuber. In this study, main antioxidant compounds in both cultivars were identified by LC-MS analysis and quantified by HPLC analysis. The result showed that three compounds (petunidin-3-O-coumaroyl-rutinoside-5-O-glucoside, pelargonidin-3-Ocoumaroyl- rutinoside-5-O-glucoside and peonidin-3-O-coumaroyl-rutinoside -5-O-glucoside) were identified as main anthocyanins in ‘Jayoung’ cultivar while only pelargonidin-3-O-coumaroyl-rutinoside-5-O-glucoside was presented as a major anthocyanin in ‘Hongyoung’ cultivar. Among chlorogenic acids, 5-O-caffeoylquinic acid was the most abundant in both cultivars. In order to evaluate hepatoprotective activity of these potatoes, the methanol extracts and their serial solvent fractions were prepared by solvent partition and XAD-7 column chromatography method and treated on hepatoma HepG2 cells of which cytotoxicity was induced by tert-butyl hydroperoxide. Among the tested samples, anthocyanin-rich fractions from both cultivars contained the strongest hepatoprotective effect on HepG2 cells. This result indicates that hepatoprotective effect of these fractions can be occurred from anthocyanin.
URI
http://pubs.kist.re.kr/handle/201004/46635
ISSN
1229229X
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KIST Publication > Article
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