Protective Effects of Chlorogenic Acid Metabolites and Coffee Extract on Oxidative and ER Stress-induced Retinal Degeneration
- Protective Effects of Chlorogenic Acid Metabolites and Coffee Extract on Oxidative and ER Stress-induced Retinal Degeneration
- 장호림; 안홍열; 조형; 김경아; 이은하; 정상훈; 이창용
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- This study was conducted to determine whether the chlorogenic acid (3-CQA), its metabolites, 3,4-dihydroxyhydrocinnamic acid (DHCA) and 3,4-dihydroxyphenylacetic acid (DOPA), and coffee extract (CE) have protective effects against N-methyl-D-aspartate (NMDA)-induced damage on the mouse retina in situ and of oxidative and ER stress-induced degeneration on a retinal precursor cells in culture. Pretreatment of R28 rat retinal neuronal cells with CE, 3-CQA, DHCA and DOPA significantly attenuated cell death caused by treatment of SIN-1 in a concentration-dependent manner.
From in vivo experiments, oral treatment of 3-CQA protected the thinning of the inner plexiform layer and increased terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling positive cells in the ganglion cell layer. The intravitreal injection of DHCA and DOPA also protect retina from NMDA-induced retinal degeneration.
In conclusion, our results indicate that 3-CQA and CE reduce retinal degeneration from oxidative and ER stress in vitro and in vivo. Also, metabolites of 3-CQA, DHCA and DOPA, might be a potential therapeutic agent for retinal degeneration.
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