CDPKs are dual-specificity protein kinases and tyrosine autophosphorylation attenuates kinase activity

Title
CDPKs are dual-specificity protein kinases and tyrosine autophosphorylation attenuates kinase activity
Authors
오만호XIa Wu김형석Jeffrey F. HarperRaymond E. ZielinskiSteven D. ClouseSteven C. Huber
Keywords
Calcium-dependent protein kinase; Tyrosine autophosphorylation; Site-directed mutagenesis; Calcium signaling; Peptide kinase activity
Issue Date
2012-11
Publisher
FEBS letters
Citation
VOL 586, NO 23, 4070-4075
Abstract
Although calcium-dependent protein kinases (CDPKs or CPKs) are classified as serine/threonine protein kinases, autophosphorylation on tyrosine residues was observed for soybean CDPKβ and several Arabidopsis isoforms (AtCPK4 and AtCPK34). We identified Ser-8, Thr-17, Tyr-24 (in the kinase domain), Ser-304, and Ser-358 as autophosphorylation sites of His6-GmCDPKβ. Overall autophosphorylation increased kinase activity with synthetic peptides, but autophosphorylation of Tyr-24 appears to attenuate kinase activity based on studies with the Y24F directed mutant. While much remains to be done, it is clear that several CDPKs are dual-specificity kinases, which raises the possibility that phosphotyrosine signaling may play a role in Ca2+/CDPK-mediated processes.
URI
http://pubs.kist.re.kr/handle/201004/46812
ISSN
00145793
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KIST Publication > Article
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