CDPKs are dual-specificity protein kinases and tyrosine autophosphorylation attenuates kinase activity
- CDPKs are dual-specificity protein kinases and tyrosine autophosphorylation attenuates kinase activity
- 오만호; XIa Wu; 김형석; Jeffrey F. Harper; Raymond E. Zielinski; Steven D. Clouse; Steven C. Huber
- Calcium-dependent protein kinase; Tyrosine autophosphorylation; Site-directed mutagenesis; Calcium signaling; Peptide kinase activity
- Issue Date
- FEBS letters
- VOL 586, NO 23, 4070-4075
- Although calcium-dependent protein kinases (CDPKs or CPKs) are classified as serine/threonine protein kinases, autophosphorylation on tyrosine residues was observed for soybean CDPKβ and several Arabidopsis isoforms (AtCPK4 and AtCPK34). We identified Ser-8, Thr-17, Tyr-24 (in the kinase domain), Ser-304, and Ser-358 as autophosphorylation sites of His6-GmCDPKβ. Overall autophosphorylation increased kinase activity with synthetic peptides, but autophosphorylation of Tyr-24 appears to attenuate kinase activity based on studies with the Y24F directed mutant. While much remains to be done, it is clear that several CDPKs are dual-specificity kinases, which raises the possibility that phosphotyrosine signaling may play a role in Ca2+/CDPK-mediated processes.
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