Development of simultaneous quantification methods of L-ascorbic acid, epigallocatechin gallate and its degradation products in storage duration by NMR spectroscopy

Title
Development of simultaneous quantification methods of L-ascorbic acid, epigallocatechin gallate and its degradation products in storage duration by NMR spectroscopy
Authors
목일균엄병헌
Keywords
qNMR; quantitative NMR; L-ascorbic acid; EGCG; Caffeine; Water-suppression technique; Stability
Issue Date
2013-10
Publisher
과학교육연구논총
Citation
VOL 29, NO 1, 11-23
Abstract
The NMR (nuclear magnetic resonance) techniques were developed for the simultaneous quantitative analysis of L-ascorbic acid (L-ascorbic acid), EGCG (epigallocatechin gallate) and its degradation products in food storage conditions. Several NMR experimental conditions including water-suppression techniques, acquisition (relaxation delay, acquisition time, etc) and processing methods were optimized for quantification of food components. Sampling method with micro NMR-tube was also developed for food components stability study with normal water (H2O) without using deuterium. The quantities of L-ascorbic acid, EGCG and its degradation products were measured by the quantitative NMR method and HPLC analysis method, respectively. The analysis results of the two methods were compared. The NMR method showed the structure of degradation products, which is not possible in HPLC methods. The NMR is quick and simple to use. In stability study of food storage, This modified NMR technique provides simultaneous and quantitative information with chemical structure of food components without time consuming separation procedure and expensive deuterium solvent.
URI
http://pubs.kist.re.kr/handle/201004/46929
ISSN
1229229X
Appears in Collections:
KIST Publication > Article
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