Development of simultaneous quantification methods of L-ascorbic acid, epigallocatechin gallate and its degradation products in storage duration by NMR spectroscopy
- Development of simultaneous quantification methods of L-ascorbic acid, epigallocatechin gallate and its degradation products in storage duration by NMR spectroscopy
- 목일균; 엄병헌
- qNMR; quantitative NMR; L-ascorbic acid; EGCG; Caffeine; Water-suppression technique; Stability
- Issue Date
- VOL 29, NO 1, 11-23
- The NMR (nuclear magnetic resonance) techniques were developed for the simultaneous
quantitative analysis of L-ascorbic acid (L-ascorbic acid), EGCG (epigallocatechin gallate) and
its degradation products in food storage conditions. Several NMR experimental conditions
including water-suppression techniques, acquisition (relaxation delay, acquisition time, etc)
and processing methods were optimized for quantification of food components. Sampling
method with micro NMR-tube was also developed for food components stability study with
normal water (H2O) without using deuterium. The quantities of L-ascorbic acid, EGCG and
its degradation products were measured by the quantitative NMR method and HPLC analysis
method, respectively. The analysis results of the two methods were compared. The NMR
method showed the structure of degradation products, which is not possible in HPLC methods. The NMR is quick and simple to use. In stability study of food storage, This modified NMR technique provides simultaneous and quantitative information with chemical structure of food components without time consuming separation procedure and expensive deuterium solvent.
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